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Researchers planning new work should review the most current literature before designing protocols, with attention to the gap between preclinical breadth and clinical-trial depth

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For example, copper ions can lead to GSH depletion through two mechanisms: the direct downregulation via the redox reaction between Cu 2+ and intracellular GSH, and the activation of a Fenton-like reaction by generating Cu + to amplify oxidative stress (Dong et al., 2020)