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The cells were cultured in a CO2 incubator (5% CO 2 , 37 °C) using Dulbecco’s modified Eagle’s medium supplemented with 10% fetal bovine serum and 1% penicillin-streptomycin

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Concentration:3%

López-Otín C, Blasco MA, Partridge L, Serrano M, Kroemer G