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Avoid repeated freeze-thaw cycles, which can degrade the peptides

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Growth was monitored via absorbance at 600 nm, and at exponential phase (OD 600 between 0.4 and 0.6), cultures were serially diluted to 10 −3 to 10 −6 OD 600 and 2 μL of the dilution were spot plated onto agar plates containing 100 µg/mL carbenicillin, 350 µg/mL clindamycin, and/or 5% w/v sucrose