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These potential benefits include preserved eGFR, reversal of bone-mineral disorder, decreased risk of progression to dialysis, reduced urinary protein loss, decreased blood phosphate, and reduced all-cause mortality

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For LC-MS analysis, samples were thawed on ice-water bath, then 40 µL of whole blood was injected into 450 µL cold extraction solvent comprising of mixture of Acetonitrile:Methanol:Milli-Q water in ratio 40:40:20 according to protocol described in Presterud et al 35