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CJC-DAC:5mg Vial + BAC Water 3ml
(8)ๅถๅคFmoc-Pro-Ala-Asp(otBu)-Asp(otBu)-Ala-Gly-Leu-Val-ๆ ่๏ผ (8) Preparation of Fmoc-Pro-Ala-Asp(otBu)-Asp(otBu)-Ala-Gly-Leu-Val-resin: ๅจๆญฅ้ชค(7)็Fmoc-Ala-Asp(otBu)-Asp(otBu)-Ala-Gly-Leu-Val-ๆ ่ไธญ๏ผๅ ๅ
ฅ่ฑๅธฝ่ฏๅ10๏ฝ50โๅๅบ5๏ฝ30ๅ้๏ผ็จ็็ฉบๆณตๆฝๅนฒ๏ผ้ๆฐๅ ๅ
ฅ่ฑๅธฝ่ฏๅ10๏ฝ50โๅๅบ10๏ฝ60ๅ้๏ผๆฝๅนฒ๏ผ็จๅผไธ้ๆดๆถค3ๆฌก๏ผDMFๆดๆถค3ๆฌก๏ผ้่ธ่ฟ็DMFๆดๆถค2ๆฌก๏ผๅ ๅ
ฅ็จDMFๆบถ่งฃ็Fmoc-Pro-OHใDIEAใTBTU/HOBtๆHBTU/HOBtๆBOP/HOBt็ๆททๅ็ฉ๏ผ10๏ฝ50โๅๅบ10๏ฝ60ๅ้๏ผ็จ็็ฉบๆณตๆฝๅนฒ๏ผ็จๅผไธ้ๆดๆถค3ๆฌก๏ผDMFๆดๆถค3ๆฌก๏ผ้่ธ่ฟ็DMFๆดๆถค2ๆฌก๏ผๆฝๅนฒ๏ผ่ทๅพFmoc-Pro-Ala-Asp(otBu)-Asp(otBu)-Ala-Gly-Leu-Val-ๆ ่๏ผ In the Fmoc-Ala-Asp(otBu)-Asp(otBu)-Ala-Gly-Leu-Val-resin of step (7), add decapping reagent 10๏ฝ50 โ and react for 5๏ฝ30 minutes, dry with vacuum pump, re- Add capping reagent and react at 10-50ยฐC for 10-60 minutes, pump dry, wash with isopropanol for 3 times, DMF for 3 times, distilled DMF for 2 times, add Fmoc-Pro-OH and DIEA dissolved in DMF , a mixture of TBTU/HOBt or HBTU/HOBt or BOP/HOBt, react at 10-50ยฐC for 10-60 minutes, dry it with a vacuum pump, wash 3 times with isopropanol, wash 3 times with DMF, wash 2 times with re-distilled DMF time, drained to obtain Fmoc-Pro-Ala-Asp (otBu)-Asp (otBu)-Ala-Gly-Leu-Val-resin