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For the purification of AtSNX1 and AtSNX2a, cell pellets were lysed by sonication in His-lysis buffer containing 50 mM Na₂HPO₄, 50 mM NaH₂PO₄ (pH 8.0), 400 mM NaCl, 20 mM imidazole, and 0.1% Tween 20

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Most studies indicate that elevated B12 is more likely a consequence of cancer or other underlying diseases rather than a cause