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Following topical application in formulation studies: Dermal penetration occurs within minutes of application, with activity detectable at target sites within 1-2 hours Small molecular size (495.58 Da) facilitates passage through stratum corneum barrier Localized concentration at application sites without significant systemic absorption Distribution pattern suggests targeting of superficial dermal layers and neuromuscular junctions The peptide’s topical bioavailability distinguishes it from larger peptides that typically require injection for biological activity